Publications
2024
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Dithiothreitol-based protein equalisation in the context of multiple myeloma: Enhancing proteomic analysis and therapeutic insights
Domingos IF, Carvalho LB, Lodeiro C, Gerivaz R, Prag G, Micaglio E, ...
Talanta, 279, 126589.
Link
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Proximal Co-Translation Facilitates Detection of Weak Protein-Protein Interactions
Kordonsky A, Gabay M, Rosinoff A, Avishid R, Florentin A, Deouell N, Prag G, ...
International Journal of Molecular Sciences, 25(20), 11099.
Link
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RING/U-box E3 protein BIR1 interacts with and ubiquitinates barley growth repressor BROAD LEAF1
Soltani O, Jöst M, Hoffie I, Hensel G, Kappel C, Prag G, McKim S, ...
Plant Physiology, 196(1), 228-243.
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CRL4DCAF1 ubiquitin ligase regulates PLK4 protein levels to prevent premature centriole duplication
Grossmann J, Kratz AS, Kordonsky A, Prag G, Hoffmann I.
Life Science Alliance, 7(6).
Link
2022
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Split-Chloramphenicol Acetyl Transferase Assay to Study Protein-Protein Interactions and Ubiquitylation in Escherichia coli
Florentin A, Kordonsky A, Yariv E, Avishid R, Efron N, Akogwu E, Prag G.
Bio-protocol, 12(17), e4497.
Link
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Polyalanine disease mutations impair UBA6-dependent ubiquitination
Amer-Sarsour F, Falik D, Berdichevsky Y, Kordonsky A, Prag G, ...
The EMBO Journal, 43(2), 250-276.
Link
2021
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Split chloramphenicol acetyl-transferase assay reveals self-ubiquitylation-dependent regulation of UBE3B
Levin-Kravets O, Kordonsky A, Shusterman A, Biswas S, Persaud A, Prag G, ...
Journal of Molecular Biology, 433(23), 167276.
Link
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Deubiquitylating enzymes in neuronal health and disease
Amer-Sarsour F, Kordonsky A, Berdichevsky Y, Prag G, Ashkenazi A.
Cell Death & Disease, 12(1), 120.
Link
2020
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An integrative synthetic biology approach to interrogating cellular ubiquitin and ufm signaling
Li C, Han T, Guo R, Chen P, Peng C, Prag G, Hu R.
International Journal of Molecular Sciences, 21(12), 4231.
Link
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Ubiquitin signaling and degradation of aggregate-prone proteins
Galves M, Rathi R, Prag G, Ashkenazi A.
Trends in Biochemical Sciences, 44(10), 872-884.
Link
2019
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Remodeling membrane binding by mono-ubiquitylation
Tanner N, Kleifeld O, Nachman I, Prag G.
Biomolecules, 9(8), 325.
Link
2018
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E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins
Levin-Kravets O, Keren-Kaplan T, Attali I, Sharon I, Tanner N, Shapira D, Prag G.
In: The Ubiquitin Proteasome System: Methods and Protocols, 155-166.
Link
2017
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Ubiquitylation‐dependent oligomerization regulates activity of Nedd4 ligases Ilan Attali, William Sam Tobelaim, Avinash Persaud, Khatereh Motamedchaboki, Kobi J Simpson‐Lavy, Bayan Mashahreh, Olga Levin‐Kravets, Tal Keren‐Kaplan, Inbar Pilzer, Martin Kupiec, Reuven Wiener, Dieter A Wolf, Daniela Rotin, and Gali Prag
Link
2016
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Structure of ubiquitylated-Rpn10 provides insight into its autoregulation mechanism
Keren-Kaplan T, Zeev Peters L, Levin-Kravets O, Attali I, Kleifeld O, Prag G, ...
Nature Communications, 7(1), 12960.
Link
2013
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Structure-based in silico identification of ubiquitin-binding domains provides insights into the ALIX-V: ubiquitin complex and retrovirus budding
Keren-Kaplan T, Attali I, Estrin M, Kuo LS, Farkash E, Prag G, ...
The EMBO Journal, 32(4), 538-551.
Link
2012
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Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria
Keren-Kaplan T, Attali I, Motamedchaboki K, Davis BA, Tanner N, Prag G, ...
The EMBO Journal, 31(2), 378-390.
Link
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Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10
Keren-Kaplan T, Prag G.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F68(2), 131-135.
Link
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Purification and crystallization of yeast Ent1 ENTH domain
Tanner N, Prag G.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F68(4), 462-466.
Link
2011
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The hetero-hexameric nature of a chloroplast AAA+ FtsH protease contributes to its thermodynamic stability
Moldavski O, Levin-Kravets O, Ziv T, Adam Z, Prag G.
PLoS One, 6(4), e19541.
Link
2008
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A phage display system designed to detect and study protein-protein interactions
Bair CL, Oppenheim A, Trostel A, Prag G, Adhya S.
Molecular Microbiology, 67(4), 719-728.
Link
2007
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The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting
Prag G, Watson H, Kim YC, Beach BM, Ghirlando R, Hummer G, Hurley JH.
Developmental Cell, 12(6), 973-986.
Link
2006
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Ubiquitin-binding domains
Hurley JH, Lee S, Prag G.
Biochemical Journal, 399(3), 361-372.
Link
2004
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Structure of the ESCRT-II endosomal trafficking complex
Hierro A, Sun J, Rusnak AS, Kim J, Prag G, Emr SD, Hurley JH.
Nature, 431(7005), 221-225.
Link
2003
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Mechanism of ubiquitin recognition by the CUE domain of Vps9p
Prag G, Misra S, Jones EA, Ghirlando R, Davies BA, Horazdovsky BF, Hurley JH.
Cell, 113(5), 609-620.
Link
2001
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High Resolution Structural Analyses of Mutant Chitinase A Complexes with Substrates Provide New Insight into the Mechanism of Catalysis
Papanikolau Y, Prag G, Tavlas G, Vorgias CE, Oppenheim AB, Petratos K.
Biochemistry, 40(38), 11338-11343.
Link
2000
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Structures of chitobiase mutants complexed with the substrate di-N-acetyl-D-glucosamine: the catalytic role of the conserved acidic pair, aspartate 539 and glutamate 540
Prag G, Papanikolau Y, Tavlas G, Vorgias CE, Petratos K, Oppenheim AB.
Journal of Molecular Biology, 300(3), 611-617.
Link
1998
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Participation of IHF and a distant UP element in the stimulation of the phage λ PL promoter
Giladi H, Koby S, Prag G, Engelhorn M, Geiselmann J, Oppenheim AB.
Molecular Microbiology, 30(2), 443-451.
Link
1997
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Structural principles of prokaryotic gene regulatory proteins and the evolution of repressors and gene activators
Prag G, Greenberg S, Oppenheim AB.
Molecular Microbiology, 26(3), 619-620.
Link